real time quantitative polymerase chain reaction rt qpcr analysis  (Bio-Rad)

Journal: Hypertension

Article Title: Epididymal Fat-Derived Sympathoexcitatory Signals Exacerbate Neurogenic Hypertension in Obese Male Mice Exposed to Early Life Stress

doi: 10.1161/hypertensionaha.121.17298

Figure Lengend Snippet: Figure 5. Obese MSEW male mice display increased serotonin content in epididymal eWAT. A, RT-qPCR profiler showing significant increases in trytophan hydroxylase 1. B, Serotonin content is eWAT determined by ELISA. Data were analyzed by the Student t test. Data were analyzed by 2-way ANOVA followed by Tukey multiple comparisons post hoc test when considering low fat (LF)–high fat (HF; Table SIV). Data were reported as mean±SEM. n=5 to 8 per group. Bdkrb1 indicates bradykinin receptor, beta 1; Bdkrb2, bradykinin receptor, beta 2; Htr2a, hydroxytryptamine (serotonin) receptor 2A; IL1β, interleukin 1 beta; IL6, interleukin-6; Lepr, leptin receptor; Ngf, nerve growth factor; NOX4, NADPH oxidase 4; p47phox (Ncf1), neutrophil cytosolic factor 1; Tnf, tumor necrosis factor; Tph1, tryptophan hydroxylase 1; and TrpV1, transient receptor potential cation channel, subfamily V, member 1. eWAT indicates epididymal white adipose tissue; MSEW, maternal separation early weaning; and RT-qPCR, Real Time quantitative Reverse Transcription Polymerase Chain Reaction. *P<0.05 vs control.

Article Snippet: Frozen tissue (n=5–8 per group) was used to extract mRNA as reported previously.28 A custom-designed Real Time quantitative Reverse Transcription Polymerase Chain Reaction (RT-qPCR) array (Bio-Rad PrimePCR; Bio-Rad Laboratories, Inc) included the following targets: Tph1 (tryptophan hydroxylase 1), Htr2a (hydroxytryptamine [serotonin] receptor 2A), TrpV1, Ngf (nerve growth factor), Bdkrb1 (bradykinin receptor, beta 1), Bdkrb2 (bradykinin receptor, beta 2), NOX4 (NADPH oxidase 4), p47phox, Ilb1 (interleukin 1 beta), Tnf (tumor necrosis factor), Lepr (leptin receptor), Cybb (cytochrome b-245, beta polypeptide), Ptgs2 (prostaglandin-endoperoxide synthase 2), Cy2c44 (cytochrome P450-family 2, subfamily c-polypeptide 44), VEGFa (vascular endothelial growth factor A), Trpa1 (transient receptor potential cation channel, subfamily A, member 1), and IL17 (interleukin 17).

Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Polymerase Chain Reaction, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Potential implications of interleukin-7 in chronic wound healing

doi: 10.3892/etm.2016.3263

Figure Lengend Snippet: Reverse transcription-quantitative polymerase chain reaction analysis of IL-7 expression levels in clinical samples. Median IL-7 expression levels were increased in healing chronic wounds compared with non-healing chronic wounds, although no significant differences were observed. Descriptive data is presented in the table. IL-7, interleukin-7; IQR, interquartile range.

Article Snippet: The tissue biopsies were sectioned using a CM1950 cryostat (Leica Microsystems Ltd., Milton Keynes, UK) at a size of 7 µm for immunohistochemical analysis and 20 µm for use in RNA extraction and generation of cDNA for reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis, where multiple tissue sections were combined and homogenised using a hand held homogeniser (Cole-Parmer Instrument Co., Ltd., London, UK) in ice-cold total RNA isolation reagent (TRI) reagent ® (Sigma-Aldrich Co., Ltd., Irvine, UK).

Techniques: Real-time Polymerase Chain Reaction, Expressing